@article{oai:yasuda-u.repo.nii.ac.jp:00000619,
 author = {西, 博行 and 川畑, 公平 and 稲垣, 昌宣 and 荒木, 菜子 and 泉田, 莉菜 and Nishi, Hiroyuki and Kawabata, Kohei and Inagaki, Masanori and Araki, Saiko and Izumida, Rina},
 issue = {50},
 journal = {安田女子大学紀要, Journal of Yasuda Women's University},
 month = {Feb},
 note = {This paper describes the development of the quality evaluation method of herbal medicines (crude drugs) by the reversed-phase (RP) HPLC with core-shell (CS) type columns, which have a core part and a superficial porous silica part, leading to high performance (high theoretical plate number) and relatively low column pressure drops. This time, fast quality evaluation methods for Sennae Folium (senna leaf) and Rhei Rhizoma (rhubarb), both are well well-known herbal medicines containing agents for promoting bowel movements (laxatives), have been investigated. Target active ingredients contained in these herbal medicines are sennoside A and sennoside B. Referring Japanese Pharmacopoeia (JP) 18th edition, the mobile phase for HPLC and the extraction solvent were optimized. By employing a mixture of diluted acetic acid (100) (1 in 80) and acetonitrile (4:1) as a mobile phase, and a solution of sodium hydrogen carbonate (1 in 1000) as an extraction solvent, successful separation and determination of sennoside A and sennoside B in these herbal medicines were obtained. Sennoside B and sennoside A eluted in this order within 5 min, leading to a fast assay method. Further other agents having pharmacological effects in Rhei Rhizoma, rhein and emodin, were also successfully determined by the RP-HPLC with CS type columns. Obtained contents of sennoside A and B in powdered senna leaf (not less than 1.0%) and powdered rhubarb (not less than 0.25%) tested conformed with JP18 specifications.},
 pages = {319--328},
 title = {コアシェル型逆相HPLCカラムを用いた生薬成分分析　―センナ及びダイオウの品質評価法の開発―},
 year = {2022},
 yomi = {ニシ, ヒロユキ and カワバタ, コウヘイ and イナガキ, マサノリ and アラキ, サイコ and イズミダ, リナ}
}