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コアシェル型逆相HPLCカラムを用いたコルチコステロイド類の迅速一斉分離での選択性改善と外用剤の定量法開発
https://doi.org/10.24613/00000540
https://doi.org/10.24613/0000054067b1b479-4db0-4edc-84bd-306ab268a266
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
02896494049031.pdf (1.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-20 | |||||
| タイトル | ||||||
| タイトル | コアシェル型逆相HPLCカラムを用いたコルチコステロイド類の迅速一斉分離での選択性改善と外用剤の定量法開発 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Fast Simultaneous Separation of Corticosteroids by Reversed-phase HPLC with Core-shell Type Columns ―Improvement of Selectivity and Application to Assay of Formulations― | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題 | Corticosteroids, Core-shell Columns, Tetrahydrofuran, Selectivity, Biphenyl column | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.24613/00000540 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
西, 博行
× 西, 博行× 川畑, 公平× 稲垣, 昌宣× 内田, 佳那× 永田, 智沙× Hiroyuki, Nishi× Kohei, Kawabata× Masanori, Inagaki× Kana, Uchida× Chisa, Nagata |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This paper describes separation of eight corticosteroids by the reversed-phase HPLC with core-shell type columns, which have a core part and a superficial porous silica part, leading to high performance (high theoretical plate number) and relatively low column pressure drops. First typical C18 and C28 columns having 2.6 μm particle size and a linear carbon chain structure were employed with the mobile phase containing acetonitrile (ACN) or methanol (MeOH) as an organic solvent. Among eight corticosteroids, cortisone and hydrocortisone,those only differ in C=O and C-OH at position 11, were co-eluted in use of ACN, on the other hand, triamcinolone acetonide and fluosinolone acetonide, those only differ F residue at position 6, were co-eluted in use of MeOH. However, all eight corticosteroids were successfully separated within 8 min by the mobile phase with tetrahydrofuran (THF). It was found that THF is quite effective for the improvement of the selectivity in the separation of corticosteroids, as already shown in the separation between berberine and palmatine, and between codeine and dihydrocodeine. This can be explained by the solubility of THF (octadecane can be soluble in THF), leading to the straight structure of the carbon chain of C18. Next, other than the straight carbon chain type columns (C18, C28), perfluorophenyl or biphenyl type columns were employed for the separation of eight corticosteroids. In use of these planer type columns, successful separation of eight corticosteroids was obtained within 8 min in use of ACN. This means the phenyl moiety of the stationary phase is effective for the small difference in the corticosteroid structure. Finally assay methods of fluosinolone acetonide formulations were developed and applied for the determination of the active ingredient in the cream and the ointment. Fast assay was successful within 5 min. | |||||
| 書誌情報 |
ja : 安田女子大学紀要 en : Journal of Yasuda Women's University 号 49, p. 305-316, 発行日 2021-02-28 |
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| 出版者 | ||||||
| 出版者 | 安田女子大学 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0289-6494 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00242368 | |||||
